The Cellular and Molecular Imaging Core of the KUTTAM is an advanced imaging center that provides personalized assistance, training, and support on all aspects of comprehensive imaging for the basic and clinical research community. It is located in a 75 m2, shared-use facility on the Koç University Health Science Campus at the 4th floor. The Core is available to researchers only by appointment. All users must be trained before they are allowed to use the microscopes. Training and assistance in the use of the instruments and associated technical procedures is provided by appointment and in scheduled in-service training sessions. Equipment in the state-of-the-art facility includes;
Leica TCS SP8 STED 3X super-resolution system
Inverted Microscope Platform Leica DMi8
Multiphoton Microscope Leica TCS SP8 MP
Leica TCS SP8 DLS (Digital LightSheet)
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The excellent optical design of Leica TCS SP8 multiphoton (MP) system makes use of long wavelengths of fully integrated infrared (IR) excitation lasers, the high efficiency of HyD detectors, and an infrared-optimized optical transmission. These allow you to deeply penetrate into the tissue with super-sensitivity and to uncover the finest details of cellular and subcellular processes.
Keeping cells viable is crucial for obtaining artifact-free data in long-term imaging. Control and maintenance of temperature, pH and humidity as well as low light stress and stage stability are prerequisites for successful experiments. The system allows fluorescence images to be displayed, enabling both transfected living cells to be visualized as well as fluorescein conjugated dyes to be observed.
One of the most exciting developments in microscopy over the past years has been the development of super resolution microscopes which provide both lateral and axial resolution at the nanometer scale. KUTTAM’s super resolution technology uses STED (Stimulated Emission Depletion) technology and provides resolution up to 20-40 nanometers. This high degree of resolution makes it possible to reveal in-cell structures in much more detail than in a confocal microscope in conventional use. In addition, the white light laser allows high-resolution multi-color imaging and sharp viewing of different structures. The system can also be used for live imaging.
Imaging requires light, but too much light can damage your cells. Light sheet microscopy is the most gentle imaging method to date, as it reduces the overall photodamage from phototoxicity and bleaching. This automatically increases the viability of your specimen. Particularly developmental biology benefits from light sheet imaging: The combination of low light illumination and high speed acquisition allows you to follow sensitive developing organisms like a Drosophilaembryo over long time periods and to understand how tissue and organs form in real time and 3D.